Random Amplified Polymorphic DNA Markers Are Useful for Purity Determination of Tomato Hybrids
نویسندگان
چکیده
منابع مشابه
Random Amplified Polymorphic DNA (RAPD) Markers
Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...
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Cashmere is only produced from Maraz (Kurdi) goat breed in Iraqi Kurdistan Region. The objective of this study was to assess the genetic diversity of different Maraz color types, black goat and Shami breeds using random amplified polymorphic DNA (RAPD) markers. Fourteen primers were used and nine out of them were selected based on their number of bands (NB) and polymorphic characteristics. Thes...
متن کاملOptimizing reproducibility evaluation for random amplified polymorphic DNA markers.
The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice for all individuals, a few randomly chose...
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sex determination in pistacia as a dioecious plant is economically desirable. identification of the male and female plants has a great value for pistacia yield production. in the present study, leaf samples from four female plants from cultivars of pistacia including akbary, akbar aghaei, fandoghi, and kalleh ghochi as well as male plants were used for rapd pcr amplification. among 32 primers w...
متن کاملMethod to improve reliability of random-amplified polymorphic DNA markers.
Random-amplified polymorphic DNA (RAPD) markers (8) are a powerful tool for genome analysis (7). Nevertheless, the difficulties to achieve a high pattern repeatability represent a major drawback for the routine implementation of RAPD markers. Amplification products obtained depend on various factors, such as the concentration of the reaction mixture constituents, the source of Taq DNA polymeras...
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ژورنال
عنوان ژورنال: HortScience
سال: 1995
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.30.2.377